Journal of Pediatric Surgery
Volume 38, Issue 1 , Pages 21-24 , January 2003

Isolation and culture of adult and fetal rabbit bladder smooth muscle cells and their interaction with biopolymers

Presented at the 49th Annual Congress of the British Association of Paediatric Surgeons, Cambridge, England, July 23-26, 2002.

  • Image Result

    Fetal and adult SMCs numbers at 48th and 96th hours.

    Fetal and adult SMCs numbers at 48th and 96th hours.

  • Image Result

    Cell growth experiments performed by the neutral red uptake assay. The indicated polymers were incubated for 1 hour with 1 mg/mL fibronectin or 10% FCS containing medium before seeding with (A) 48,000

    Cell growth experiments performed by the neutral red uptake assay. The indicated polymers were incubated for 1 hour with 1 mg/mL fibronectin or 10% FCS containing medium before seeding with (A) 48,000 per well adult, (B) 69,000 per well fetus SMCs. The assay was performed after 7 days in culture.

 Address reprint requests to B. Haluk Guvenc, MD, Kocaeli University Medical Faculty, Department of Pediatric Surgery, Kocaeli, 41900, Turkey.

PII: S0022-3468(02)63011-8

doi: 10.1053/jpsu.2003.50003

Journal of Pediatric Surgery
Volume 38, Issue 1 , Pages 21-24 , January 2003