Journal of Pediatric Surgery
Volume 42, Issue 2 , Pages 300-304 , February 2007

The effect of age on colocalization of acetylcholinesterase and nicotinamide adenine dinucleotide phosphate diaphorase staining in enteric neurons in an experimental model

  • Tamas Cserni

      Affiliations

    • Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland
    • Pediatric Surgical Department, Medical Health Science Centre, University Of Debrecen, Nagyerdei Krt. 98 4012, Hungary
    • ,
  • Sri Paran

      Affiliations

    • Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland
    • ,
  • Prem Puri

      Affiliations

    • Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland
    • Corresponding Author InformationCorresponding author. Tel.: +353 14096420; fax: +353 14550201.

References 

  1. Porter AJ, Wattchow DA, Brookes SJ, et al. Cholinergic and nitrergic interneurones in the myenteric plexus of the human colon. Gut. 2002;51:70–75
  2. Rolle U, Nemeth L, Puri P. Nitrergic innervation of the normal gut and in motility disorders of childhood. J Pediatr Surg. 2002;37:551–567
  3. Rolle U, Chertin B, Nemeth L, et al. Demonstration of nitrergic and cholinergic innervation in whole-mount preparations of rabbit, pig, and human upper urinary tract. Pediatr Surg Int. 2002;18:315–318
  4. Furness JB. Pharmacology of transmission and sites of drug action in the enteric nervous system. In:  Furness JB editors. The enteric nervous system. Massachusets: Blackwell Publishing Inc; 2006;p. 103
  5. Puri P, Rolle U. Variant Hirschsprung's disease. Semin Pediatr Surg. 2004;13:293–299
  6. Montedonico S, Sri T, Paran M, et al. Developmental changes in submucosal nitrergic neurons in the porcine distal colon. J Pediatr Surg. 2006;41:1029–1035
  7. Karnovsky MJ, Roots L. A direct colouring thiocholine method for cholinesterase. J Hystochem Cytochem. 1965;12:219–221
  8. Kiernan JA. Histological and histochemical methods: theory and practice. New York: Pergamon Press; 1990;
  9. Wester T, O'Briain S, Puri P. Morphometric aspects of the submucous plexus in whole-mount preparations of normal human distal colon. J Pediatr Surg. 1998;33:619–622
  10. De Ridder E, Weyns A. Classification of the enteric nerve cells of the porcine small intestine into two subpopulations using enzyme histochemical techniques. Microsc Res Tech. 1994;29:103–111
  11. Aimi Y, Kimura H, Kinoshita T, et al. Histochemical localization of nitric oxide synthase in rat enteric nervous system. Neuroscience. 1993;53:553–560
  12. Costa M, Brookes SJ, Steele PA, et al. Neurochemical classification of myenteric neurons in the guinea-pig ileum. Neuroscience. 1996;75:949–967
  13. Furness JB. Types of neurons in the enteric nervous system. J Auton Nerv Syst. 2000;81(1-3):87–96Review
  14. Atoji Y, Mizutani K, Yamamoto Y. Suzuki Y Innervation of the pigeon oviduct: correlation of NADPH diaphorase with acetylcholinesterase, tyrosine hydroxylase, and neuropeptides. Auton Neurosci. 2000;84:1–7
  15. Atoji Y, Yamamoto Y, Suzuki Y. Innervation of NADPH diaphorase-containing neurons correlated with acetylcholinesterase, tyrosine hydroxylase, and neuropeptides in the pigeon cloaca. J Anat. 2001;198:181–188
  16. Neunlist M, Aubert P, Toquet C, et al. Changes in chemical coding of myenteric neurones in ulcerative colitis. Gut. 2003;52:84–90
  17. Schneider J, Jehle EC, Starlinger MJ, et al. Neurotransmitter coding of enteric neurones in the submucous plexus is changed in non-inflamed rectum of patients with Crohn's disease. Neurogastroenterol Motil. 2001;13:255–264

 Presented at the British Association of Paediatric Surgeons 53rd Annual International Congress, Stockholm, Sweden, July 18-22, 2006.

PII: S0022-3468(06)00759-7

doi: 10.1016/j.jpedsurg.2006.10.031

Journal of Pediatric Surgery
Volume 42, Issue 2 , Pages 300-304 , February 2007

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