Protective effect of erythropoietin on torsion/detorsion injury in rat model

Bakan, Vedat; Çıralık, Harun; Tolun, Fatma İnanaç; Atlı, Yalçın; Mil, Ayhan; Öztürk, Şenol

doi:10.1016/j.jpedsurg.2009.02.071

« Previous Next »Journal of Pediatric Surgery
Volume 44, Issue 10 , Pages 1988-1994, October 2009

Protective effect of erythropoietin on torsion/detorsion injury in rat model

Vedat Bakan
- Faculty of Medicine, Department of Pediatric Surgery, Sutcu Imam University, Kahramanmaras 46100, Turkey
- Corresponding author. KSÜ Tıp Fakültesi Çocuk Cerrahisi AD Kahramanmaraş, Turkey. Tel.: +90 344 221 23 37; fax: +90 344 221 23 71.
Harun Çıralık
- Faculty of Medicine, Department of Pathology, Sutcu Imam University, Kahramanmaras 46100, Turkey
Fatma İnanaç Tolun
- Faculty of Medicine, Department of Biochemistry, Sutcu Imam University, Kahramanmaras 46100, Turkey
Yalçın Atlı
- Faculty of Medicine, Department of Biochemistry, Sutcu Imam University, Kahramanmaras 46100, Turkey
Ayhan Mil
- Faculty of Medicine, Department of Pediatric Surgery, Sutcu Imam University, Kahramanmaras 46100, Turkey
Şenol Öztürk
- Faculty of Medicine, Department of Pediatric Surgery, Sutcu Imam University, Kahramanmaras 46100, Turkey

Received 21 December 2008; received in revised form 12 February 2009; accepted 13 February 2009.

Abstract

Purpose

The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats.

Methods

Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D₁), 3 hours ischemia and 1 hour reperfusion; group III (torsion/detorsion 2, T/D₂), 3 hours ischemia and 48 hours reperfusion; group IV (erythropoietin 1, EPO₁), 3 hours ischemia, 1 hour reperfusion, and a single dose of EPO; and group V (erythropoietin 2, EPO₂), 3 hours ischemia, 48 hours reperfusion, and 2 doses of EPO. Malondialdehyde (MDA) and nitric oxide (NO) levels and activities of superoxide dismutase and catalase were measured. Tissue damage to ovarian tissue was scored by histologic examination. Data were compared among groups with parametric tests.

Results

The MDA levels in the S and EPO groups were significantly lower than the T/D groups (P < .001). Catalase and superoxide dismutase activities, and NO levels in the S and EPO groups were significantly higher than in the T/D groups (P < .05). Ovarian tissue damage in the S and EPO groups was significantly less than in the T/D groups (P < .05). Levels of all biochemical markers and ovarian tissue damage scores were similar among the S, EPO₁, and EPO₂ groups (P > .05).