Journal of Pediatric Surgery
Volume 45, Issue 3 , Pages 549-554, March 2010

Characterization of pediatric Wilms' tumor using Raman and fluorescence spectroscopies

  • Chad A. Lieber

      Affiliations

    • CHOC Research Institute, Children's Hospital of Orange County, Orange, CA 92868, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1 714 516 4257; fax: +1 714 516 4318.
    • ,
  • Mustafa H. Kabeer

      Affiliations

    • Department of Pediatric Surgery, Children's Hospital of Orange County, Orange, CA 92868, USA
    • Department of Surgery, University of California at Irvine Medical Center, Irvine, CA 92697, USA

Received 24 February 2009; received in revised form 9 July 2009; accepted 10 July 2009.

Abstract 

Purpose

Raman spectroscopy has been successfully demonstrated as an effective tool for tissue characterization and diagnosis, but nearly all studies have interrogated adult tissues and diseases. In this study, we demonstrate the application of Raman spectroscopy and its background autofluorescence for pediatric Wilms' tumor diagnosis.

Methods

Eight tumors were measured in this study, along with matched normal kidney tissue in 6 cases. Spectral comparisons were drawn, and diagnostic use was assessed using both the Raman spectral features as well as the inherent tissue fluorescence.

Results

The fluorescent background spectra were able to discriminate normal kidney from Wilms' tumor with 81% sensitivity and 100% specificity. The Raman spectra obtained 93% sensitivity and 100% specificity.

Conclusions

This pilot study shows that both autofluorescence and Raman spectra provide diagnostic use in discriminating Wilms' tumor from normal kidney. These techniques may be used individually or in tandem to develop a real-time intraoperative screening and diagnostic device.

Key words: Automated diagnosis, Raman spectroscopy, Fluorescence, Optical, Pediatric cancer

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PII: S0022-3468(09)00587-9

doi:10.1016/j.jpedsurg.2009.07.030

Journal of Pediatric Surgery
Volume 45, Issue 3 , Pages 549-554, March 2010

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