Lack of intestinal pacemaker (C-KIT-positive) cells in infantile hypertrophic pyloric stenosis☆

Abstract 

The pathogenesis of infantile hypertrophic pyloric stenosis (IHPS) is not well understood. Recent studies have shown that the protooncogene c-kit is essential for the development or maintenance of autonomic gut motility, and also show that the c-kit gene protein product (C-KIT) positive cells in the mammalian gut are responsible for intestinal pacemaker activity. This study examines cells in the pyloric muscles of 23 patients (16 with IHPS, 7 controls) for the presence of the C-KIT (C-KIT⁺), using immunohistochemical techniques with antihuman C-KIT sera. In the controls, many C-KIT immunoreactive (IR⁺) cells were observed in the muscle layers. The myenteric plexuses were demarcated by a moderate number of C-KIT-IR⁺ cells. However, in the IHPS patients, C-KIT-IR were either absent or significantly reduced. No C-KIT-IR⁺ cells were found around the myenteric plexuses. These findings suggest that a lack of c-kit expression (as an indicator of intestinal pacemaker activity) in the hypertrophic pyloric smooth muscles may be an important factor in the pathogenesis of IHPS.

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