Journal of Pediatric Surgery
Volume 32, Issue 10 , Pages 1420-1423, October 1997

Abnormalities of neuropeptides and neural markers in the esophagus of fetal rats with Adriamycin-induced esophageal atresia

  • W Cheng

      Affiliations

    • Corresponding Author InformationAddress reprint requests to W. Cheng, MD, Division of Pediatric Surgery, Department of Surgery, The University of Hong Kong, Queen Mary Hospital, Pok Fu Lam Rd, Hong Kong.
    • Division of Paediatric Surgery, Department of Surgery, The University of Hong Kong Hong Kong
    • the Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, England
    • the Paediatric Surgical Unit, Institute of Child Health, London, England.
    • Department of Surgery, Great Ormond Street Hospital for Children, London, England.
    • ,
  • A.E Bishop

      Affiliations

    • Division of Paediatric Surgery, Department of Surgery, The University of Hong Kong Hong Kong
    • the Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, England
    • the Paediatric Surgical Unit, Institute of Child Health, London, England.
    • Department of Surgery, Great Ormond Street Hospital for Children, London, England.
    • ,
  • L Spitz

      Affiliations

    • Division of Paediatric Surgery, Department of Surgery, The University of Hong Kong Hong Kong
    • the Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, England
    • the Paediatric Surgical Unit, Institute of Child Health, London, England.
    • Department of Surgery, Great Ormond Street Hospital for Children, London, England.
    • ,
  • J.M Polak

      Affiliations

    • Division of Paediatric Surgery, Department of Surgery, The University of Hong Kong Hong Kong
    • the Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, England
    • the Paediatric Surgical Unit, Institute of Child Health, London, England.
    • Department of Surgery, Great Ormond Street Hospital for Children, London, England.

Abstract 

Background/Purpose: To investigate the distribution of neural markers and neuropeptides in esophageal atresia (EA).

Methods: A fetal rat model with Adriamycin-induced EA was used. The animals were divided into four groups: (1) control group, (2) saline-injected group, (3) Adriamycin administered but without the development of EA, and (4) Adriamycin-induced EA group. Specimens of the distal esophagus from each group were immunostained using antibodies to S100, protein gene product 9.5 (PGP), somatostatin, vasoactive intestine peptide (VIP), bombesin, galanin, substance P, neuropeptide Y (NPY), calcitonin gene-related product (CGRP), met-encephalin, nitric oxide synthase, and tyrosine hydroxylase.

Results: The total cross-sectional area of the distal atretic esophagus was significantly smaller than controls (P = .01), the submucosa being the component most affected (0.0465 v 0.0234 mm). Immunoreactivity for S100 and galanin were significantly elevated in the atresia group (0.0288 v 0.0079 and .001 v 0.000). In addition, there was also an increase in CGRP and Substance P in the atretic group.

Conclusion: The elevated levels of S100 and galanin could explain the disordered motility observed in patients who had esophageal atresia.

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PII: S0022-3468(97)90552-2

Journal of Pediatric Surgery
Volume 32, Issue 10 , Pages 1420-1423, October 1997

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