Journal of Pediatric Surgery
Volume 34, Issue 11 , Pages 1603-1605, November 1999

Promoting effect of estrogen on the proliferation of hemangioma vascular endothelial cells in vitro☆

  • Xianmin Xiao

      Affiliations

    • Corresponding Author InformationAddress reprint requests to Xianmin Xiao, MD, Department of Surgery, Children's Hospital, Shanghai Medical University, Shanghai 200032, China.
    • Department of Surgery, Children's Hospital, Shanghai Medical University, Shanghai, China.
    • the Institute of Radiation Medicine, Shanghai Medical University, Shanghai, China.
    • ,
  • Li Hong

      Affiliations

    • Department of Surgery, Children's Hospital, Shanghai Medical University, Shanghai, China.
    • the Institute of Radiation Medicine, Shanghai Medical University, Shanghai, China.
    • ,
  • Minli Sheng

      Affiliations

    • Department of Surgery, Children's Hospital, Shanghai Medical University, Shanghai, China.
    • the Institute of Radiation Medicine, Shanghai Medical University, Shanghai, China.

Abstract 

Purpose: This study was designed to observe whether estrogen can enhance the proliferation of hemangioma vascular endothelial cells (VECs) and, if so, the possible inhibiting effect of tamoxifen against estrogen.

Methods: Two skin hemangiomas with positive estrogen receptor staining from 2 infants were used for VECs culture. Based on different culture conditions and treatment, the subcultured VECs of passage 3 derived from a hemangioma were divided into 5 groups: group 1, control without endothelial cell growth supplement (ECGS) in medium; group 2, estradiol (E2) without ECGS; group 3, control with ECGS in medium; group 4, E2 with ECGS; group 5, E2 and 4OH-tamoxifen with ECGS. Cell counts and 3H-TdR incorporations were determined on culture days 3, 6, and 9. VECs from the other hemangioma were divided into 2 groups: group 3, control with ECGS in medium; group 4, E2 with ECGS.

Results: At the end of the 9-day study, the cell counts (×104/mL) of the 5 groups were 6.31 ± 1.24, 6.52 ± 1.08, 15.62 ± 1.88, 36.77 ± 3.96, and 6.88 ± 1.20, respectively. 3H-TdR incorporations (cpm) were 511 ± 127, 538 ± 26, 1,350 ± 67, 2,729 ± 145, and 575 ± 64, respectively. The results of the other hemangioma were similar to those of the first one. Our data showed that without ECGS in medium, E2 had no effect on the proliferation of VECs (group 1 v group 2, P > .05); with ECGS in medium, E2 yielded a 2-fold increase in the proliferation of VECs (group 3 v group 4, P < .01); when 4OH-tamoxifen was added, the proliferation of VECs was suppressed dramatically (group 4 v group 5, P < .01).

Conclusions: Estrogen in vitro can promote the proliferation of hemangioma VECs. This promoting effect of estrogen may depend on certain growth factors, which can be inhibited by tamoxifen.

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☆ Presented at the 32nd Annual Meeting of the Pacific Association of Pediatric Surgeons, Beijing, China, May 9–14, 1999.

PII: S0022-3468(99)90625-5

Journal of Pediatric Surgery
Volume 34, Issue 11 , Pages 1603-1605, November 1999

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