Journal of Pediatric Surgery
Volume 34, Issue 11 , Pages 1615-1619 , November 1999

Highly sensitive analysis for N-myc amplification in neuroblastoma based on fluorescence in situ hybridization

  • Tatsurou Tajiri

      Affiliations

    • Corresponding Author InformationAddress reprint requests to Tatsurou Tajiri, MD, Department of Pediatric Surgery, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
    • Department of Pediatric Surgery, Kyushu University, Fukuoka, Japan.
    • Department of Pathology, Kyushu University, Fukuoka, Japan.
    • ,
  • Kumiko Shono

      Affiliations

    • Department of Pediatric Surgery, Kyushu University, Fukuoka, Japan.
    • Department of Pathology, Kyushu University, Fukuoka, Japan.
    • ,
  • Yoshimitsu Fujii

      Affiliations

    • Department of Pediatric Surgery, Kyushu University, Fukuoka, Japan.
    • Department of Pathology, Kyushu University, Fukuoka, Japan.
    • ,
  • Shinichi Noguchi

      Affiliations

    • Department of Pediatric Surgery, Kyushu University, Fukuoka, Japan.
    • Department of Pathology, Kyushu University, Fukuoka, Japan.
    • ,
  • Yoshiaki Kinoshita

      Affiliations

    • Department of Pediatric Surgery, Kyushu University, Fukuoka, Japan.
    • Department of Pathology, Kyushu University, Fukuoka, Japan.
    • ,
  • Masazumi Tsuneyoshi

      Affiliations

    • Department of Pediatric Surgery, Kyushu University, Fukuoka, Japan.
    • Department of Pathology, Kyushu University, Fukuoka, Japan.
    • ,
  • Sachiyo Suita

      Affiliations

    • Department of Pediatric Surgery, Kyushu University, Fukuoka, Japan.
    • Department of Pathology, Kyushu University, Fukuoka, Japan.

References 

  1. Crist WM, Kun LE. Common solid tumors of childhood. N Engl J Med. 1991;324:461–471
  2. Fong CT, Dracopoli ND, White PS, et al.  Loss of heterozygosity for chromosome IP in human neuroblastomas: Correlation with N-myc amplification. In: ed 2. Proc Natl Acad Sci USA. 49:1989;p. 1095–1098
  3. Bourhis J, De Vathaire F, Wilson GD, et al.  Combined analysis of DNA ploidy index and N-myc genomic content in neuroblastoma. Cancer Res. 1991;51:33–36
  4. Look AT, Hayes FA, Shuster JJ, et al.  Clinical relevance of tumor cell ploidy and N-myc gene amplification in childhood neuroblastoma: A pediatric oncology group study. J Clin Oncol. 1991;9:581–591
  5. Shapiro DN, Valentine MB, Rowe ST, et al.  Detection of N-myc gene amplification by flurescence in situ hybridization. Am J Pathol. 1993;142:1339–1346
  6. Tsuda T, Obara M, Hirano H. Analysis of N-myc amplification in relation to disease stage and histologic types in human neuroblastomas. Cancer. 1987;60:820–826
  7. Brodeur GM, Seeger RC, Schwab M, et al.  Amplification of N-myc in untreated human neuroblastomas correlates with advanced disease stage. Science. 1984;224:1121–1124
  8. Cohn SL, Look AT, Johsi VV, et al.  Lack of correlation of N-myc gene amplification with prognosis in localized neuroblastoma: A Pediatric Oncology Group Study. Cancer Res. 1995;55:721–726
  9. Kaneko M, Nishihira H, Mugishima H, et al.  Stratification of treatment of stage 4 neuroblastoma patients based on N-myc amplification status. Med Pediatr Oncol. 1998;31:1–7
  10. Lorenzana AN, Zielenska M, Thorner P, et al.  Heterogeneity of MYCN amplification on a child with stroma-rich neuroblastoma (ganglioneuroblastoma). Pediatr Pathol Lab Med. 1997;17:875–883
  11. Strehl S, Ambros PF. Fluorescence in situ hybridization combined with immunohistochemistry for highly sensitive detection of chromosome 1 aberrations in neuroblastoma. Cytogenet Cell Genet. 1993;63:24–28

☆ Presented at the 32nd Annual Meeting of the Pacific Association of Pediatric Surgeons, Beijing, China, May 9–14, 1999.

PII: S0022-3468(99)90628-0

Journal of Pediatric Surgery
Volume 34, Issue 11 , Pages 1615-1619 , November 1999

Article Tools