Abstract
There is increasing evidence to suggest that reactive oxygen and nitrogen species
play a role in the pathogenesis of renal ischemia-reperfusion (I/R) injury. This study
was designed to determine the possible protective effects of trapidil treatment against
oxidative and nitrosative tissue injury of kidney induced by I/R.
A renal I/R injury was induced by a left renal pedicle occlusion by ischemia for 45
minutes, followed by 1 hour of reperfusion with contralateral nephrectomy in I/R and
I/R + trapidil groups. Trapidil (8 mg/kg intravenously) was administrated immediately
before reperfusion phase. At the end of the reperfusion period, rats were killed.
Then, renal tissue samples were taken for biochemical analysis and histopathological
evaluation, and blood samples were obtained to determinate serum urea, aspartate aminotransferase
(AST), and tumor necrosis factor–α (TNF-α) levels. Ischemia-reperfusion injury caused significant increases in myeloperoxidase
activity and malondialdehyde and 3-nitrotyrozine levels in renal tissue and elevated
serum urea, AST, and TNF-α levels. In addition, severe deterioration of renal morphology was seen in the I/R
group. Trapidil treatment significantly reduced in biochemical parameters, as well
as serum urea, AST, and TNF-α levels. Furthermore, renal tissue injury was markedly attenuated with trapidil treatment.
These data suggest that reactive oxygen species and reactive nitrogen species play a causal role in I/R-induced renal tissue, and trapidil has a renoprotective
effect against oxidative and nitrosative kidney damage.
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