Abstract
Purpose
The pathophysiology of testicular torsion-detorsion is an ischemia-reperfusion injury
caused by overgeneration of reactive oxygen species (ROS). This study aimed to investigate
the effect of rutin, a well-known antioxidant, on testicular ischemia-reperfusion
injury.
Methods
Sixty male Sprague-Dawley rats were randomly divided into 3 groups, each containing
20 rats. Rats in the control group underwent a sham operation of the left testis.
In the torsion-detorsion group, the left testis was rotated 720° for 2 hours. Rats
in the treatment group received the same surgical procedure as the torsion-detorsion
group, but rutin was administered intravenously at the time of detorsion. Bilateral
orchiectomy was performed on half of the rats in each experimental group at 4 hours
after detorsion for measurement of malondialdehyde, an indicator of intratesticular
ROS content, and for evaluation of superoxide dismutase and catalase, which are endogenous
antioxidant enzymes. Orchiectomy was performed on the remaining rats at 3 months after
detorsion for analysis of testicular spermatogenesis.
Results
Unilateral testicular torsion-detorsion caused a significant increase in malondialdehyde
level and caused significant decreases in superoxide dismutase, catalase activities,
and spermatogenesis in ipsilateral testes. The rats treated with rutin had a significant
decrease in malondialdehyde level and had significant increases in superoxide dismutase,
catalase activities, and spermatogenesis in ipsilateral testes, compared with torsion-detorsion
group.
Conclusions
Rutin protects testes from ischemia-reperfusion injury. The protective effect of rutin
may be caused by scavenging ROS by increasing superoxide dismutase and catalase activities.
Key words
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Article info
Publication history
Accepted:
September 20,
2010
Received in revised form:
September 20,
2010
Received:
August 23,
2010
Identification
Copyright
© 2011 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
