Abstract
Purpose
We sought to determine whether amniotic cell profiles correlate quantitatively with
neural tube defect (NTD) type and/or size.
Methods
Sprague–Dawley fetuses exposed to retinoic acid (n = 61) underwent amniotic fluid sample procurement before term. Samples were analyzed
by flow cytometry for the presence of cells concomitantly expressing Nestin and Sox-2
(neural stem cells, aNSCs), and cells concomitantly expressing CD29 and CD44 (mesenchymal
stem cells, aMSCs). Statistical analysis included ANOVA and post-hoc Bonferroni adjusted
comparisons (P < 0.05).
Results
There was a statistically significant increase in the proportion of aNSCs in fetuses
with spina bifida (6.78% ± 1.87%) when compared to those with exencephaly (0.64% ± 0.23%) or with both spina bifida and exencephaly (0.22% ± 0.09%). Conversely, there was a statistically significant decrease in the proportion
of aMSCs in fetuses with exencephaly, either isolated (1.09% ± 0.42%) or in combination defects (2.37% ± 0.63%) when compared with normal fetuses (8.83% ± 1.38%). In fetuses with isolated exencephaly, there was a statistically significant
inverse correlation between the proportion of aNSCs and defect size.
Conclusions
The proportions of neural and mesenchymal stem cells in the amniotic fluid correlate
with the type and size of experimental NTDs. Targeted quantitative amniotic cell profiling
may become a useful diagnostic tool in the prenatal evaluation of these anomalies.
Key words
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Article info
Publication history
Accepted:
March 8,
2013
Received:
February 21,
2013
Identification
Copyright
© 2013 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
