Abstract
Background
We have previously demonstrated that heparin-binding EGF-like growth factor (HB-EGF)
administration protects the intestines from ischemia/reperfusion (I/R) injury in vivo.
We have also shown that HB-EGF promotes mesenchymal stem cell (MSC) proliferation
and migration in vitro. The goals of the current study were to examine the effects
of HB-EGF and both bone marrow (BM)- and amniotic fluid (AF)-derived MSC on intestinal
I/R injury in vivo.
Materials and Methods
MSC were isolated from pan-EGFP mice, expanded, and purified. Pluripotency was confirmed
by induced differentiation. Mice were subjected to terminal ileum I/R and received
either: (1) no therapy; (2) HB-EGF; (3) BM-MSC; (4) HB-EGF + BM-MSC; (5) AF-MSC; or (6) HB-EGF + AF-MSC. MSC engraftment, histologic injury, and intestinal permeability were quantified.
Results
There was increased MSC engraftment into injured compared to uninjured intestine for
all experimental groups, with significantly increased engraftment for AF-MSC + HB-EGF compared to AF-MSC alone. Administration of HB-EGF and MSC improved intestinal
histology and intestinal permeability after I/R injury. The greatest improvement was
with combined administration of HB-EGF + AF-MSC.
Conclusions
Both HB-EGF alone and MSC alone can protect the intestines from I/R injury, with synergistic
efficacy occurring when HB-EGF and AF-MSC are administered together.
Key words
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Article info
Publication history
Accepted:
March 8,
2013
Received:
February 19,
2013
Identification
Copyright
© 2013 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
