Abstract
Background
We have previously demonstrated that heparin-binding EGF-like growth factor (HB-EGF)
and mesenchymal stem cell (MSC) administration protect the intestines from ischemia/reperfusion
(I/R) injury in vivo, with amniotic fluid-derived MSC (AF-MSC) being more efficacious than bone marrow-derived
MSC (BM-MSC). The goal of the current study was to determine whether the protective
effects of HB-EGF were from direct effects on MSC or via alternative mechanisms.
Methods
Murine MSC were transfected with an HB-EGF plasmid or control plasmid by electroporation.
Mice were subjected to segmental intestinal I/R injury and received either BM-MSC
or AF-MSC either with or without exogenous HB-EGF, or BM-MSC or AF-MSC that endogenously
over-expressed HB-EGF. MSC engraftment, intestinal histologic injury, and intestinal
permeability were quantified.
Results
There was increased MSC engraftment into injured compared to uninjured intestine.
HB-EGF increased AF-MSC engraftment into injured intestine. Administration of HB-EGF
and MSC improved intestinal histology and intestinal permeability after I/R injury,
with AF-MSC being most efficacious. The effect of HB-EGF on MSC was similar when the
growth factor was administered exogenously, or when it was overexpressed endogenously.
Conclusions
The effect of HB-EGF on AF-MSC was similar with both exogenous administration and
endogenous overexpression of the growth factor, implying that HB-EGF has a direct
effect on AF-MSC. This information may assist in guiding potential future AF-MSC-based
therapies for patients at risk of intestinal ischemic injuries.
Key words
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Article Info
Publication History
Published online: February 03, 2014
Accepted:
January 27,
2014
Received:
January 16,
2014
Identification
Copyright
© 2014 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
